期刊论文

中文

天然产物研究与开发

1001-6880

2013

25

1

71-75

10.3969/j.issn.1001-6880.2013.01.018

Foundation Item： Hunan Provincial Natural Science Foundation （ 11 JJ2042 ） Science of Pharmaceutical Analysis of Twelfth Five-Year Key Discipline Projects of Hunan University of Chinese Medicine In- novation Platform and Open Foundation Project of Higher Colleges of Hunan Province （11 K048 ） Changsha City Science and Technology Bureau Key Projects（ Kl104104-21 ）

本校为第一机构

建立反相高效液相色谱法(RP-HPLC)测定chiisanoside和chiisanogenin的定量分析方法,利用最优色谱条件对十种中韩五加属植物叶中的三萜chiisanoside和chiisanogenin进行定量分析.研究结果表明,最佳色谱条件为:ODS-C18色谱柱(250mm×4.6mm,5μm);流动相:乙腈-水(55:45);检测波长:205 nm;柱温:40℃;流速:1.0 mL/min.Chiisanoside和chiisanogenin线性范围和回归方程分别为16.5～ 181.5 μg/mL,Y=5498356X-9738(r=0.9995)和4.88～78.0 μg/mL,Y=5753131X-8289 (r=0.9979),加样回收率分别为98.87％(标准偏差为1.14％)和98.83％(标准偏差为0.72％).

A RP-HPLC method for simultaneous quantitative analysis of chiisanoside and chiisanogenin was developed and optimized. Chiisanoside and chiisanogenin from leaves of ten kinds of Acanthopanax Miq. plants were quantitatively analyzed by the developed method. The best condition was established as follows: the separation was performed with an ODS-C_(18) column (250 mm × 4. 6 mm, 5 μm) at 40 ℃, and the mobile phase was CH_3CN-H_2O (55∶ 45). Chiisanoside and chiisanogenin were detected at 205 nm, the flow rate was 1 mL/min. The linear ranges of c...