期刊论文

中文

天然产物研究与开发

1001-6880

2014

26

11

1815-1818

10.16333/j.1001-6880.2014.11.010

本校为其他机构

以高效液相色谱法测定神安颗粒中五味子醇甲含量,建立神安颗粒多指标质量控制体系中脂溶性成分的控制指标。采用 Kromasil C_(18)色谱柱,流动相为甲醇-水 (60∶ 40),检测波长为250 nm,流速为1. 0 mL / min,柱温为室温,进样量为10 μL。结果五味子醇甲进样量在0. 15 ~ 6. 0 μg 范围内与峰面积积分值线性关系良好 (R~2= 1),平均回收率为97. 38% (RSD = 2. 05%),测定神安颗粒中五味子醇甲含量约为1. 30 mg / g。本方法简便、快捷、专属性强、重现性好,可用于神安颗粒天麻苷类成分的质量控制。

The objective of this study was to develop a high performance liquid chromatography （HPLC） method for the determination of schisandrin in Shenan granules and hence to establish a quality control system of Shenan granules using liposoluble constituent as the controlling index. The chromatographic separation was performed on a Kromasil C18 column, the mobile phases consisted of methanol-water （60： 40）, the detection wavelength was 250 nm, flow rate was 1.0 mL/min,column temperature was room temperature, and the injection volume was 10μL. Th...