期刊论文

Anti-influenza Activity of Acalypha australis L. Ethanol Extract and Its Mechanism

中文

天然产物研究与开发

1001-6880

2016

28

11

1706-1711,1751

10.16333/j.1001-6880.2016.11.004

湖南省“十二五”重点学科中药学资助(2014) 湖南省***卫生人才“225”工程项目(2013)

本校为第一机构

研究铁苋菜乙醇提取物对感染流感病毒小鼠肺部、流感病毒神经氨酸酶活性及其抗氧化活性的影响。采用鼻腔接种建立流感病毒感染的小鼠肺炎模型,观察各组小鼠的肺部病理变化。并以2-(4-methylumbelliferyl) -α-D-N-acetylneuraminic acid(MUNANA)为底物,检测铁苋菜乙醇提取物高、中和低剂量组对流感病毒神经氨酸酶活性的影响。利用NO法、DPPH法和β-胡萝卜素漂白法测定了铁苋菜乙醇提取物抗氧化活性。结果显示,在动物实验中,与空白组相比,模型组小鼠肺泡、细支气管等正常的组织结构遭到破坏。与模型组相比,奥司他韦组和铁苋菜乙醇提取物高、中、低剂量组均能明显改善感染的小鼠肺部病理损伤,且改...

To investigate the effects of Acalypha australis L. ethanol extract on influenza virus-induced lung inflammation in mice, activity of influenza virus neuraminidase and its antioxidant activities, the influenza virus-infected lung inflammation model mice was established by intranasal inoculation, followed by the observation of pathological changes of lung tissue in mice. The inhibition activity of high,medium and low dosage groups on influenza virus neuraminidase were detected by the substrate of 2-(4-methylumbelliferyl) -α-D-N-acetylneuraminic...