期刊论文

Study on chemical constituents and their anti-inflammatory activity from ethyl acetate extract of fruits of Acanthopanax henryi (Oliv.) Harms

中文

天然产物研究与开发

1001-6880

2020

32

3

427-434

10.16333/j.1001-6880.2020.3.011

赣南医学院博士科研启动基金(QD201905) 湖南省自然科学基金(2019JJ40223) 湖南中医药大学生物工程重点学科资助(校行科字[2018]3号)。

本校为其他机构

基于脂多糖(LPS)诱导的小胶质细胞BV2为生物活性导向模型首次研究糙叶五加Acanthopanax henryi(Oliv.) Harms果实的化学成分.采用正反相硅胶柱色谱、凝胶柱色谱、制备薄层、制备液相及重结晶等方法进行分离纯化,利用波谱分析结合理化性质鉴定化合物的结构.从糙叶五加果实甲醇提取物的乙酸乙酯萃取部位中分离得到18个化合物,分别鉴定为5-羟甲基-2-糠醛(1)、5-羟基麦芽酚(2)、原儿茶酸(3)、6-甲氧基-7-羟基香豆素(4)、山柰酚-3-O-β-D-葡萄糖苷(5)、山奈酚-3-芸香苷(6)、(-)-松脂醇4-O-β-D-吡喃葡萄糖苷(7)、(+)-simplexoside (8)、(-)-芝麻素(9)、松香(10)、苯甲基-β-D-吡喃葡萄糖苷-6'-O-乙酸...

To study the chemical constituents from the fruits of Acanthopanax henryi (Oliv.) Harms based on LPS-induced microglia BV2 as the bioactivity guided model.The compounds were isolated and purified by NP-and RP-silica gel and Sephadex LH-20 column chromatography,as well as Prep-TLC,Prep-HPLC,and recrystallization methods.Their structures were identified on the basis of their physicochemical properties and spectroscopic data.As a result,eighteen compounds were obtained from A.henryi and their chemical structures were identified as 5-hydroxymethyl-...