期刊论文

Study on the isolation,identification and content determination of anti-inflammatory components in Tuniuxi

中文

天然产物研究与开发

1001-6880

2020

32

7

1171-1181

10.16333/j.1001-6880.2020.7.012

湖南省教育厅优秀青年基金(18B251) 湖南省食品药品监督管理局食品药品安全科技项目(湘食药科R201806) 永州市科技创新及应用研究项目(永财企指[2019]2号) 湖南中医药大学中药学一流建设学科(校行科字[2018]3号)。

本校为第一机构

本研究建立脂多糖诱导的巨噬细胞炎症模型,结合层析法对土牛膝抗炎活性成分进行分离,并用NMR、MS以及与对照品比较,对得到的化合物进行结构鉴定.结果 表明粗毛牛膝醇提取物抗炎效果最佳,其反相层析50％、70％及100％甲醇洗脱物抗炎活性高和细胞毒性低,从50％～70％甲醇洗脱物分离鉴定出9种单体化合物:β-蜕皮甾酮(1)、牛膝甾酮(2)、水龙骨甾酮B(3)、N-反式阿魏酰酪胺(4)、N-顺式阿魏酰酪胺(5)、N-顺式阿魏酰-3-甲氧基酪胺(6)、N-反式阿魏酰-3-甲氧基酪胺(7)、竹节参皂苷Ⅳa(8)、5,2'-二甲氧基-6-甲氧甲基-7-羟基-异黄酮(10);100％甲醇洗脱物鉴定出主要成分竹节参皂苷Ⅰ(9).在LPS +25 μM单体浓度...

The inflammation macrophages (RAW 264.7) model induced by lipopolysaccharide (LPS) with nitric oxide (NO) release as an index was established to investigate the anti-inflammatory components in different varieties of Tuniuxi and column chromatography was used to isolate the active compounds from Achyranthes aspera Linnacus methanol extract.The chemical structures of the active components were deduced based on the nuclear magnetic resonance (NMR) and mass spectrometry (MS) data analysis and comparing with reference substances.The methanol extract...