期刊论文

Mechanism research of Danshen-Chuanxiong in treating Alzheimer's disease based on network pharmacology

中文

天然产物研究与开发

1001-6880

2021

33

08

1382-1390

10.16333/j.1001-6880.2021.8.014

国家自然科学基金（81373702）;国家自然科学基金（81874462）； 重庆市中医药重点学科（中医基础理论）建设项目（渝中医[2021]16号）；

本校为其他机构

利用网络药理学方法研究丹参-川芎药对治疗阿尔茨海默病（AD）的作用机制。经数据库查找及文献检索,获得丹参和川芎的有效成分,利用STITCH与TCMSP数据库得到有效成分作用靶点,并构建药物-靶标网络、PPI网络,利用Metascape数据库对丹参-川芎作用靶点进行GO分析以及KEGG代谢通路富集分析。最后利用MTT实验、ELISA法、流式细胞仪和荧光定量PCR实验验证丹参-川芎对PC12细胞的保护作用。结果显示筛选出丹参-川芎药对25个活性成分和作用于AD的靶点105个,GO分析相关度前20个靶点分子功能,前10条重要信号通路。细胞实验证实了丹参-川芎可显著提高PC12细胞的细胞存活率和拮抗PC12细胞的炎症反应及降低PC1...

To study the mechanism of Danshen-Chuanxiong(DS-CX)in the treatment of Alzheimer's disease(AD)by using network pharmacology.Through database search and literature retrieval,the effective ingredients of DS and CX were obtained,and the targets of active ingredients were obtained through STITCH and TCMSP databases.And the drugs-targets network and PPI network were built.Then,gene ontology(GO)analysis and KEGG metabolic pathway enrichment analysis were performed on the action targets of DS-CX were performed using Metascape database.Finally,MTT,ELIS...