期刊论文

Study on mechanism of Radix et Rhizoma Rhei in treating Alzheimer's disease based on network pharmacology

中文

天然产物研究与开发

1001-6880

2021

33

01

127-136

10.16333/j.1001-6880.2021.1.016

国家自然科学基金（81373702,81874462）；

本校为第一机构

基于网络药理学探讨大黄治疗阿尔茨海默病（AD）的作用机制。借助TCMSP数据库及Uniprot数据库筛选出大黄有效成分及靶点基因。通过Drugbank、Dis Ge NET和TTD数据库筛选出阿尔茨海默病的靶点基因;成分靶点与疾病靶点映射后使用Cytoscape 3.7.1软件构建药物有效成分-靶点蛋白相互作用网络,使用String数据库绘制靶点蛋白-靶点蛋白相互作用网络;对靶点蛋白利用Metascape数据库进行GO分析和KEGG分析,最后采用MTT实验、ELISA法、比色法和荧光实时定量PCR对网络药理学主要分析结果进行验证。研究共筛选出大黄的有效成分17个,对应靶点276个,与阿尔茨海默病相关靶点共107个,KEGG相关信号通路前10条,GO分...

To explore the mechanism of Radix et Rhizoma Rhei in the treatment of Alzheimer's disease(AD) based on network pharmacology.The effective components and target genes of Radix et Rhizoma Rhei were screened by TCMSP database and Uniprot database.The target genes of AD were screened by Dragbank,Dis Ge NET and TTD databases.After mapping the component target with the disease target point,Cytoscape 3.7.1 software was used to construct the drug active component-target protein interaction network,and at the same time,the String database was used to dr...