期刊论文

中文

天然产物研究与开发

1001-6880

2022

34

5

723-738

10.16333/j.1001-6880.2022.5.001

国家重点研发计划中医药现代化研究重点专项（2017YFC1701900）； 长沙市重点研发计划（kq2004054）； 中央引导地方科技发展专项子项（2019XF5076）； 湖南中医药大学中药学一流学科项目（校行科字[2018]3号）；

本校为第一机构

山香圆叶具有较好的临床疗效,但其挥发油的化学成分和生物活性尚未深入研究.本文应用超临界萃取法提取其挥发油,采用GC-MS对其小极性物质进行全面分析(甲酯化和非甲酯化),并对其中等极性物质进行LC-MS分析,采用了 DPPH法和ABTS法测定了其抗氧化活性,采用LPS诱导RAW 264.7细胞模型探索山香圆叶挥发油的抗炎活性.GC-MS非甲酯化和甲酯化分别鉴定了 38和42个化合物,主要为亚油酸、γ-谷甾醇、角鲨烯、2,2'-亚甲基双-(4-甲基-6-叔丁基苯酚)、反-9-十八碳烯酸、棕榈酸等物质.LC-MS的正负离子模式下,共鉴定出256个化合物,主要为萜类、苯丙素类、酚类、黄酮类、生物碱类、香豆素及其衍生物类、脂肪酰类、...

Turpiniae Folium is widely used as a functional food or medicine in China.However,little investigations are performed on the chemical composition and biological activity of its volatile oil.In this work,Turpiniae Folium oil extracts(Supercritical extraction)were systematically characterized for the first time.The low polar substances were identified by GC-MS(methyl esterification and non-methylesterification),and LC-MS method was applied to conduct analysis of the intermediate polar substances.Also,the anti-oxidant(DPPH and ABTS method)and anti...