In this study,the chemical fingerprints of roots,leaves,fruits and stems of Macleaya cordata were established by HPLC,and the contents of protopine,allocryptine,sanguinarine and chelerythrine were determined;orthogonal partial least squares discriminant analysis(OPLS-DA)was used to evaluate the quality of roots,leaves and fruits of M.cordata.The fingerprint results showed that 17 batches of root samples were calibrated with 10 common peaks,14 batches of leaf samples were calibrated with 9 common peaks,14 batches of fruit samples were calibrated with 9 common peaks,and 10 batches of stem sample...