期刊论文

中文

微生物学通报

0253-2654

2014

41

9

1764-1770

10.13344/j.microbiol.china.130819

长沙市科技局项目（No.k1307020-31） 湖南省培育发展战略性新兴产业专项引导资金（长财企指[2012]号）

本校为第一机构

【目的】探讨超微铁皮石斛对脾虚便秘小鼠肠道乳酸杆菌多样性的影响,为疗效提供依据。【方法】制备小鼠脾虚便秘模型,灌胃铁皮石斛传统汤剂、超微50%量汤剂治疗,采集肠道内容物提取肠道微生物宏基因组DNA,用乳酸杆菌特异引物PCR后进行ARDRA分析。【结果】结果显示,正常组、铁皮石斛传统汤剂组和超微50%量汤剂组的OTUs、Shannon指数和Brillouin指数相同,且均大于模型组;铁皮石斛超微50%量汤剂组与正常组乳酸杆菌群落结构的相似性系数最大,为0.333 3,其次是模型组为0.181 8,传统汤剂组最小,为0.166 7;聚类分析和主成分分析结果显示,小鼠肠道乳酸杆菌多样性受脾虚便秘造模影响,发生改变,铁皮石斛两...

[Objective] To provide the basis for its treatment, the effect of ultra-micro powder Dendrobium officinale on the molecular diversity of intestinal Lactobacillus in mice with spleen-deficiency constipation was studied. [Methods] The mice with spleen-deficiency constipation in treatment groups were respectively given the traditional decoction of Dendrobium officinale and50% dose of ultra-micro powder Dendrobium officinale. The metagenome DNA of intestinalmicroflora was extracted from intestinal contents. Then ARDRA analysis was made after PCR by specific primer of Lactobacillus. [Results] The r...