期刊论文

Expression of ILT-4 mRNA in peripheral blood mononuclear cell of liver cancer patients

中文

武警后勤学院学报(医学版)

2095-3720

2012

21

12

955-957

10.3969/j.issn.2095-3720.2012.12.003

本校为第一机构

[目的]检测外周血单个核细胞(peripheral blood mononuclear cell,PBMC)免疫球蛋白转录子4(immunoglobulin-liketranscript-4,ILT-4)mRNA在肝癌患者中的表达,并探讨其在肿瘤发生发展中的作用.[方法]以β-actin为内参照,实时荧光定量RT-PCR方法检测肝癌患者和健康体检者外周血单个核细胞ILT-4 mRNA的表达.[结果]正常对照组ILT-4 mRNA表达水平(0.450±0.216)显著低于肝癌组(0.887±0.253,P＜0.05),肝癌患者组在不同分期之间的表达差异无统计学意义(P＞0.05),低分化(1.061±0.126)显著高于高、中分化(0.693±0.215,P＜0.05).[结论]肝癌患者外周血PBMC ILT-4mRNA表达水平上调,检测PBMC的ILT-4基因表...

[Objective]To investigate the expression level of ILT-4 in PBMC of liver cancer patients and its clinical significance. [Method]The mRNA of ILT-4 in PBMC were detected by real-time relative quantitative PCR method in 38 patients with liver cancer and 40 healthy individuals. [Result]The relative expression level of ILT-4 in PBMC in liver cancer group（0.887±0.253） was significantly higher than healthy group（0.450±0.216, P 0.05 ）. The relative expression level of ILT-4 in PBMC in poorly differentiated group（1.061±0.126）was significantly h...