会议论文

中文

2012

268-274

2012年朱文峰学术思想研讨会暨中医诊断师资班30周年纪念大会

2012年朱文峰学术思想研讨会暨中医诊断师资班30周年纪念大会论文集

2012-10-01

长沙

中国中西医结合学会

中华中医药学会

本校为第一机构

目的:比较健脾法、补肾法与解毒法对慢性乙肝病毒(HBV)感染患者外周血DCs介导的T淋巴细胞的影响。方法:分别收集10例HBV慢性感染免疫耐受期、免疫清除期患者和8例健康人的抗凝外周血,分离外周血单个核细胞(PBMCs),体外培养DCs,用大鼠健脾、补肾、解毒药物血浆进行干预,并建立DCs、T细胞相互作用的模型,检测T细胞的表面分子CD8~+CD28~+、CD8~+PD-1受体的表达。结果:经补肾

　　Objective:To compare the effect of TCM decoctions(Sijunzi Tang, Liuweidihuang Tang and Ganluxiaodn Tang)on T lymphocytes of people with chronic HBV infection,which were mediated by peripheral blood DCs.Methods:Tan patients in immune tolerance stage and eight patients in immune clearance stage with chronic hepatitis B virus infection and eight healthy controls were included in this study.DCs were differentiated from PBMCs which were isolated from their peripheral blood ,incubated and induced then,the DCs were interfered by plasma of medicine...