期刊论文

Wang, C.

中文

针刺研究

1000-0607

2010

35

2

113-117

10.13702/j.1000-0607.2010.02.006

湖南省自然科学基金重点课题（No.00JJY1006）； 湖南省教育厅课题（No.08C633）；

本校为第一且通讯机构

目的:观察电针"内关"穴对实验性心肌缺血再灌注损伤大鼠心肌细胞内钙超载及内源性保护物质一氧化氮(NO)、一氧化氮合酶(NOS)的影响,为针刺防治心血管疾病及经脉脏腑相关理论提供实验依据.方法:50只Wistar雄性大鼠随机分为假手术组、缺血再灌注模型组、电针内关组、电针列缺组、电针合谷组.采用冠脉结扎法建立心肌缺血再灌注模型,各电针组于冠脉结扎前后各电针20 min(30 Hz/100 Hz,2～4 mA).硝酸还原酶比色法检测各组心肌组织NO、NOS的含量,在Fluo-3/AM染色后于激光共聚焦显微镜下检测心肌细胞内Ca2+荧光强度.结果:与假手术组比较,模型组心肌组织NOS含量明显降低(P

OBJECTIVE: To observe the effect of electroacupuncture (EA) on contents of myocardial nitric oxide (NO), nitric oxide synthase (NOS), and intracellular Ca2+ levels in experimental myocardial ischemia-reperfusion injury (MI/RI) rats, so as to provide some evidence for acupuncture treatment of cardiovascular disorders and for the correlation between the acupoints and the internal organs. METHODS: Fifty male Wistar rats were randomly divided into sham-operation (sham), model, Neiguan (PC 6), Lieque (LU 7), and Hegu (LI 4) groups (n = 10/group). MI/RI model was established by occlusion of the desc...