期刊论文

Hong, J.

中文

针刺研究

1000-0607

2011

36

3

157-163,192

10.13702/j.1000-0607.2011.03.001

30772707:国家自然科学基金 20070541003:高等学校博士学科点专项科研基金

本校为第一且通讯机构

目的:探讨艾灸血浆对胃黏膜细胞凋亡的影响，揭示艾灸对胃黏膜细胞的保护作用及其信号转导机制。方法:24名健康人随机等分为艾灸穴位组和艾灸非穴位组，分别艾灸中脘、关元和足三里穴及非穴位对照点各10d。将人体胃黏膜上皮(GES-1)细胞分为空白组、模型组、艾灸穴位血浆组、艾灸非穴位血浆组，采用含8％乙醇的培养液造成GES-1细胞损伤模型，分别加以预先提取的灸前人体血浆、艾灸穴位和非穴位血浆。流式细胞技术检测细胞凋亡率；Western blot法检测细胞内热休克蛋白70(HSP 70)、第二个线粒体来源的胱氨酸酶激活物(Smac)、凋亡诱导因子(AIF)的表达；细胞免疫化学法检测半胱氨酸天冬酸蛋白酶-3、9...

OBJECTIVE: To observe the effect of plasma derived from healthy volunteers undergoing moxibustion (moxibustion plasma) on alchol-injured human gastric epithelial GES-1 cells in vitro, and expression of heat shock protein 70 (HSP 70, cell apoptosis inhibitory protein), apoptosis inducing factor (AIF), Smac (a mitochondrial protein), and Caspase 3 and Caspase 9 (the latter 3 proteins are also involved in cell apoptosis) in order to study its mechanisms underlying protecting gastric mucous membrane. METHODS: Twenty-four healthy volunteer subjects (half men and half women) were randomized into acu...