期刊论文

中文

针刺研究

1000-0607

2018

43

6

360-364

10.13702/j.1000-0607.170409

(2014 CB 543102):国家重点基础研究发展“973”计划项目 (CX 2017 B 454):2017年湖南省研究生创新项目

本校为第一机构

目的:观察针刺不同经脉穴位对失眠大鼠松果体褪黑素(MT)含量及视交叉上核(SCN)内褪黑素受体1(MT_1)、褪黑素受体2(MT_2)mRNA表达的影响,探讨固定局部穴位配伍不同经脉穴位之间作用的差异。方法:雄性SD大鼠随机分为空白组、模型组、百会+神门组、百会+三阴交组和百会+非经非穴组,每组12只。采用腹腔注射对氯苯丙氨酸混悬液建立失眠模型。治疗组每日针刺治疗1次,连续治疗7 d。运用免疫组织化学法检测大鼠松果体MT的表达量,运用实时荧光定量PCR法检测大鼠SCN内MT_1、 MT_2 mRNA的表达量。结果:与空白组比较,模型组大鼠松果体MT灰度值升高,SCN内MT_1、MT_2 mRNA表达量降低(P

Objective To observe the effect of manual acupuncture stimulation of paired "Baihui" (GV 20) + "Shenmen" (HT 7),GV 20 + "Sanyinjiao" (SP 6),and GV 20 + non-acupoint on expression of melatonine (MT) and suprachiasmatic melatonin receptor 1 (MT_1) and melatonin receptor 2 (MT2) mRNAs in insomnia rats, so as to explore their action difference and the underlying mechanism in improving insomnia. Methods Male SD rats were randomly divided into normal control (n = 12),model (n = 8), GV 20 + HT 7(n = 12),GV 20 +SP 6(n = 11), and GV 20 + non-acupoint (n...