期刊论文

Electroacupuncture and moxibustion pretreatments reduce cardiomyocyte apoptosis and autophagy inrats with myocardial ischemia/reperfusion injury

中文

针刺研究

1000-0607

2019

44

1

31-36

10.13702/j.1000-0607.170671

国家自然科学基金面上项目(No.81574080) 湖南省高校创新平台开放基金项目(No.15k093) 湖南省中管局课题(No.201521).

本校为第一机构

目的:观察针灸预处理对心肌缺血大鼠心肌细胞凋亡指数和自噬相关蛋白LC3表达的影响,探讨针灸预处理对心肌缺血大鼠心肌细胞自噬能力的预保护作用.方法:SD大鼠随机分为假手术组、模型组、缺血预适应(IP)组、电针组、艾灸组,每组8只.电针组及艾灸组造模前分别电针或艾灸“内关”穴,每天1次,每次20 min,连续7d.采用冠状动脉结扎术复制心肌缺血模型.电镜观察左心室缺血区组织病理形态学及心肌细胞自噬体的变化,TUNEL法检测心肌细胞凋亡情况及凋亡指数,Western blot法检测LC3蛋白在心肌组织中的表达情况.结果:假手术组心肌细胞形态完整,排列整齐,线粒体较完整,可见少量自噬空泡;与假手术组相比,模型组...

OBJECTIVE: To observe the effect of "Neiguan" (PC6)-electroacupuncture (EA) or moxibustion (Moxi) pretreatment on myocardial apoptosis and expression of autophagy related proteins light chain (LC) 3- and LC3- in rats with myocardial ischemia/reperfusion injury (MIRI), so as to explore their mechanisms underlying improvement of MIRI. METHODS: Forty SD rats (half male and half female) were randomly divided into sham operation, model, ischemic preconditioning (IP), EA and Moxi groups (n8 in each group). EA (10 Hz/50 Hz, 1 mA) or Moxi (ignited moxa stick) was respectively applied to bilateral "Nei...