期刊论文

Acupoint catgut embedding improves dysmenorrhea by suppressing activation of NLRP3 inflammasomes in uterine tissues in primary dysmenorrhea rats

中文

针刺研究

1000-0607

2019

44

7

481-485

10.13702/j.1000-0607.180508

国家自然科学基金(No.81503385) 2018年国家级大学生创新创业训练计划项目(No.201810541016) 2018年湖南省大学生创新创业训练计划项目(No.433).

本校为第一机构

目的:观察穴位埋线对原发性痛经(PD)大鼠子宫组织核苷酸结合寡聚化结构域受体3(NLRP3)炎性小体及其下游因子白细胞介素1β(IL-1β)、白细胞介素18(IL-18)表达的影响,探讨穴位埋线治疗PD的可能机制.方法:雌性SD大鼠随机分为正常组、模型组、埋线组、西药组,每组10只.苯甲酸雌二醇联合缩宫素皮下注射建立PD大鼠模型.埋线组于造模第1天及造模第5天对"关元""三阴交"穴进行埋线治疗;西药组采用芬必得灌胃治疗,每日1次,共10 d.于第11天比较各组大鼠30 min内扭体次数;HE染色法观察大鼠子宫病理形态学的变化,并进行病理损伤评分;Western blot法检测子宫组织NLRP3、半胱氨酰天冬氨酸特异性蛋白酶1(caspas...

Objective To observe the effect of acupoint catgut embedding on the expression of nucleotide-binding oligomerization domain (NOD)-like receptor protein 3 (NLRP3) inflammasome, interleukin (IL)-1β and IL-18 in the uterine tissue of primary dysmenorrhea (PD) rats, so as to explore its underlying mechanisms in improving PD. Methods Forty female SD rats were randomly divided into control, model, acupoint catgut embedding and medication groups (n=10 in each group). The PD model was established by subcutaneous injection of Estradiol Benzoate (0.5 mg...