期刊论文

Effect of acupoint catgut embedding on levels of PG-related factors and NF-κB proteins in ute-rine tissues of rats with primary dysmenorrhea

中文

针刺研究

1000-0607

2020

45

7

548-551,556

10.13702/j.1000-0607.190687

国家自然科学基金项目(No.81503385) 2018年湖南省大学生创新创业训练计划项目(No.433) 2018年度湖南中医药大学中西医结合一流学科开放基金项目(No.2018ZXYJH35) 湖南中医药大学“十三五”一级学科基础医学建设项目(No.06)。

本校为第一机构

观察穴位埋线对原发性痛经(PD)大鼠子宫组织中前列腺素F_(2α)(PGF_(2α))、环氧化酶-2(COX-2)和核转录因子κB (NF-κB)的影响,探讨穴位埋线治疗PD的可能机制。方法:SD雌性大鼠随机分为正常组、模型组、穴位埋线组、西药组,每组10只。采用苯甲酸雌二醇联合缩宫素建立PD大鼠模型。穴位埋线组大鼠于造模第1天及造模第5天予"关元""三阴交"埋线治疗;西药组大鼠予125 mg/100 mL布洛芬灌胃(0.8 mL/只)治疗,连续给药10 d。第11天,各组大鼠腹腔注射缩宫素(2 U/只)后,ELISA法检测子宫组织中PGF_(2α)含量;Western blot法检测子宫组织中COX-2、NF-κB p65、磷酸化NF-κB (phospho-NF-κB p65)的蛋白表达水...

OBJECTIVE: To observe the effect of acupoint catgut embedding on levels of prostaglandin F(2α)(PGF(2α)), cyclooxygenase-2(COX-2) and nuclear factor kappa B(NF-κB) in the uteruses of rats with primary dysmenorrhea (PD), so as to explore its mechanisms underlying improvement of PD. METHODS: Forty female SD rats were randomly divided into normal, model, acupoint catgut embedment and medication groups, with 10 rats in each group. The PD model was established by subcutaneous injection of estradiol benzoate (0.5 mg on the 1(st) day, and 0.2 mg the...