期刊论文

Effect of electroacupuncture on urodynamics of neurogenic bladder and PACAP/cAMP/PKA signaling pathway in detrusor tissue of rats after suprasacral spinal cord injury

中文

针刺研究

1000-0607

2021

46

09

728-734+741

10.13702/j.1000-0607.200880

国家自然科学基金面上项目（No.81473753、81874510）； 湖南省自然科学基金青年项目（No.2019JJ50437）； 湖南省中医药管理局科研计划项目重点课题（No.201704）；

本校为第一机构

目的:观察电针对骶上脊髓损伤后神经源性膀胱尿流动力学及逼尿肌组织中垂体腺苷酸环化酶激活肽(PACAP)/环磷酸腺苷(cAMP)/蛋白激酶A(PKA)信号通路的影响,探讨电针改善骶上脊髓损伤休克期后逼尿肌反射亢进型膀胱功能的可能机制。方法:60只雌性SD大鼠按随机数字表法分为空白组12只、假手术组12只、造模组36只。造模组采用改良胸(T)10脊髓横断法制作神经源性膀胱模型。将造模成功的大鼠随机分为模型组12只、电针治疗组12只。电针治疗组造模后第19天于\"次髎\"\"中极\"\"三阴交\"给予电针干预,20 min/次,1次/d,连续治疗7 d。干预结束后各组行尿流动力学检测,HE染色法观察膀胱逼尿肌组织病理形态学变...

OBJECTIVE: To observe the effect of electroacupuncture (EA) on urodynamics of neurogenic bladder and pituitary adenylate cyclase activating peptide(PACAP)/cyclic adenosine monophosphate (cAMP)/protein kinase A(PKA) signaling pathway in detrusor tissue of rats after suprasacral spinal cord injury (SCI), so as to explore its possible mechanism in improving detrusor hyperreflexia bladder function after shock stage of suprasacral SCI. METHODS: Female SD rats were randomly divided into control, sham operation, model and EA groups, with 12 rats in each group. T10 spinal cord transection (SCT) was pe...