期刊论文

中文

湖南中医药大学学报

1674-070X

2009

29

2

23-25

10.3969/j.issn.1674-070X.2009.02.008

湖南省自然科学基金资助项目（07JJ6056）； 湖南省教育厅科研资助项目（07C475）；

本校为第一机构

目的 探讨丹龙醒脑片对大鼠脑缺血再灌注海马区细胞凋亡指数(AI)、Fas/FasL、TNF-α表达的影响.方法 运用大脑中动脉栓塞再通法建立脑缺血再灌注模型,将动物随机分为假手术组、模型组、丹龙醒脑片组、尼莫地平组,采用原位末端标记法(TUNEL)检测凋亡神经细胞.采用免疫组织化学法,观察海马区Fas/FasL、TNF-α的表达.结果 丹龙醒脑片能显著减轻神经细胞的损伤;假手术组有一定量的Fas/FasL、TNF-α表达,缺血后Fas/FasL、TNF-α迅速增加,丹龙醒脑片组显著抑制Fas/FasL、TNF-α的表达,模型组和丹龙醒脑片组、尼莫地平组比较,差异均具有统计学意义(P＜0.05).结论 抑制由缺血再灌注损伤诱导的Fas/FasL、...

Objective To investigate the effect of Danlong Xingnao tablet （DLXNT） on expression of AI, Fas/FasL and TNF-α after cerebral ischemic-reperfusion in hippocampus in rats. Methods The model of cerebral ischemia-reperfusion was induced by middle cerebral artery occlusion （MCAO） and recanalization. The rats were randomly divided into four groups including sham-operation, control, DLXNT and nimodipine groups. The expression of AI, Fas/FasL, TNF-α in hippocampus were detected with TUNEL and immunohistochemistry. Results DLXNT could reduce obvio...