期刊论文

中文

中国人兽共患病学报

1002-2694

2011

27

11

1016-1020

10.3969/j.issn.1002-2694.2011.11.016

国家自然科学基金（30570952）资助项目；

本校为其他机构

目的　构建携带SV40LT基因的重组腺病毒表达载体，制备具有感染力的重组腺病毒，观察其转染日本血吸虫（Schistosoma japonicum,Sj）童虫细胞后的表达情况。方法　采用体外连接法构建好的携带SV40LT基因的重组腺病毒质粒(AdHu5-SV40LT)转化Stb12感受态菌，获得重组腺病毒质粒后，经Pac I酶切线性化后转染293A细胞，获得出重组腺病毒（AdHu5-SV40LT）。将重组腺病毒感染Sj童虫细胞，采用RT-PCR和免疫组织化学检测SV40LT基因的表达情况。结果　重组腺病毒载体质粒可转染293A细胞并可在293A细胞内进行有效的复制；提取病毒DNA进行PCR检测证实含有 SV40LT目的基因。以重组腺病毒能感染Sj童虫细胞，经...

In order to pack the recombinant adenovirus vector carrying SV40LT gene and to study the effect of the recombinant adenovirus on transfecting schistosomulum cells,The constructed recombinant adenovirus plasmid was re-transformed into competent cells Stbl2,and identified by restriction enzyme digestion.The right recombinant adenovirus plasmid was transfected into 293A cells using Lipofectine 2000.The recombinant adenovirus was amplified,and then identified by polymerase chain reaction（PCR）.The effect of the recombinant adenovirus transfecting ...