期刊论文

Tian Xue-Feic

中文

数字中医药(英文)

2096-479X

2020

3

2

67-79

10.1016/j.dcmed.2020.06.002

We thank for the funding support from the National Natural Science Foundation of China (No. 81473617), the Science and Technology Department of Hunan Province (No. 2017SK50310) and the Hunan Education Department's Science & Research Project (No. 16K066).

本校为第一机构

目的通过观察蜈蚣提取物（CSE）对人肝癌细胞Hep G2及裸鼠肝癌原位移植瘤的影响,探讨其抗肝癌的机制。方法 Hep G2细胞分为CSE250（250μg/m L）处理组、CSE500（500μg/m L）处理组、5-FU（5-氟尿嘧啶）处理组和对照组,采用酶解丙酮沉淀法分离纯化CSE并作用于人肝癌细胞HepG2,CCK8法检测细胞增殖抑制,流式细胞术检测细胞周期变化。Western blot检测CSE处理后HepG2细胞信号传导与转录激活因子3（STAT3）信号通路相关蛋白表达变化。制备人肝癌细胞裸鼠原位移植瘤模型,随机分为模型组、5-FU组、CSE10组[10mg/（kg·d）]和CSE50组[50mg/（kg·d）],每组12只,观察裸鼠原位移植瘤瘤体体积及质量变化,...

Objective: To observe the effects of Centipede Scolopendra extraction (CSE) on human liver cancer HepG2 cells and the nude mouse tumor model of liver orthotopic transplantation, and to explore the anti-liver cancer mechanism of the extract. Methods: HepG2 cells were respectively treated with CSE250 (250 μg/mL), CSE500 (500 μg/mL) and 5-FU, and control group was established. An enzymatic hydrolysis and acetone precipitation method was used to separate and purify CSE, which was then used to treat HepG2 cells. The CCK8 assay was used to detect t...