期刊论文

中文

时珍国医国药

1008-0805

2017

28

1

88-91

10.3969/j.issn.1008-0805.2017.01.030

湖南省教育厅优秀青年项目（No.15B179） 湖南中医药大学方剂学省级重点学科开放基金项目（No.2015-02） 湖南中医药大学博士科研启动项目（No.1001-017） 湖南中医药大学中医诊断学国家重点学科开放基金（No.2015ZYZD19）

本校为第一机构

目的观察甘草含药血清对胃黏膜上皮细胞(GES - 1)迁移、增殖及细胞内多胺(腐胺、精脒、精胺)含量的影响,探讨甘草促进胃黏膜损伤修复的作用机制。方法枪头(Tips)划痕法建立GES - 1细胞损伤迁移模型,NIH Image J软件统计分析细胞迁移率; MTT法检测细胞增殖;柱前衍生高效液相色谱法测定细胞内多胺含量。结果10%甘草含药血清可加速GES - 1细胞迁移(与空白对照血清组比较P < 0.01);在损伤后24 h或36 h,10%甘草含药血清可促进GES - 1细胞增殖(与空白对照血清组比较P < 0.01); 10%甘草含药血清可提高胃黏膜上皮损伤修复过程细胞内腐胺、精脒和精胺含量(与空白对照组血清组比较P < 0.05,P < 0.01) 。结...

Objective To study the effects of Radix Glycyrrhizae medicated serum on migration,proliferation,and polyamines(putrescine,spermidine,and spermine) content of IEC - 6 cells,so as to explore the mechanism of Radix Glycyrrhizae in promoting gastric mucosal repair. Methods cell injury model was induced by tips,cell migration rate was determined by NIH Image J software; cell proliferation was detected by MTT assay; polyamines content was measured by pre - column derivation high performance liquid chromatography(HPLC). Results 10% Radix Glycyrrhizae medicated serum significantly promote GES - 1 migr...