期刊论文

Lu, FG

英文

Microchimica Acta

0026-3672

2019

186

4

216

10.1007/s00604-019-3332-x

We would like to thank National Natural Science Foundation (81774126, 81803964), Key Project Funded by Hunan traditional Chinese medicine administration (201711), Open Project Funded by Hunan Provincial Key Laboratory (20160214), Open Project Funded by Innovation Platform of Education department of Hunan Province (17K067), Doctor start-up Foundation of Hunan University of Chinese Medicine (9982-1001019), Science and Technology Innovation Team in Colleges and Universities in Hunan Province《Chinese traditional medicine for treatment of infectious diseases》(No: 15) and Key Subjects of Hunan University of Chinese Medicine《Basic medicine》(NO.1) for the financial support. We also thank Gabrielle David, PhD, from Liwen Bianji, Edanz Group China (www.liwenbianji.cn/ac), for editing the English text of a draft of this manuscript.

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A highly sensitive and selective fluorometric method is described for determination of mercury(II). It is based on (a) the use of graphene oxide (GO) acting as a quencher of the fluoresence of the carboxy-fluorescein (FAM), and (b) of Hg(II)-triggered cleavage of the newly formed nucleic acid sequences harbored blunt 3′-hydroxyl termini by exonuclease III (Exo III) that leads to signal amplification. Two DNA probes are used, viz. a capture probe (CP) and a help probe; HP) that is partially complementary. In the absence of Hg(II), the FAM-label...