期刊论文

Study of Daniongxingnao Recipe on Proliferation of Neural Stem Cells and Expressions of β-catenin, Wnt-3a in Cerebral Ischemia-Reperfusion Injury Model Rats and its Mechanism

中文

湖南中医药大学学报

1674-070X

2015

35

1

7-10

10.3969/j.issn.1674-070X.2015.01.002.007.04

81373702:国家自然科学基金 81202632:国家自然科学基金 12B097:湖南省教育厅科学研究项目

本校为第一机构

目的探究丹龙醒脑方对脑缺血再灌注损伤大鼠海马区内源性神经干细胞增殖与β-catenin、Wnt-3a表达的影响。方法将60只雄性SD大鼠随机分为假手术组、模型组、依达拉奉组（西药组）、丹龙醒脑方小剂量组（丹小组）、丹龙醒脑方大剂量组（丹大组）,线栓法制备局灶性脑缺血再灌注（I/R）损伤模型,再灌注7 d后取大鼠缺血侧脑组织。采用Brd U掺入法记录海马齿状回颗粒下区Brd U阳性细胞数目;采用RT-q PCR法检测海马总β-catenin、Wnt-3a m RNA表达;采用Western Blot法检测海马总β-catenin、胞浆β-catenin、Wnt-3a蛋白表达。结果与假手术组比较,其余各组Brd U阳性细胞数、β-catenin、Wnt-3a蛋白及m ...

Objective To study the effect of Danlongxingnao recipe on proliferation of endogenous neural stem cells and expressions of β-catenin, Wnt-3a in hippocampus of cerebral ischemia-reperfusion injury model rats. Methods 60 male SD rats were randomly divided into sham operation group, model group, the edaravone group （western medicine group）, Danlongxingnao recipe small dose group （Dan Xiao group）, Danlongxingnao recipe high-dose group （Dan Da group）. The focal cerebral ischemia reperfusion （I/R） injury models were prepared by suture method...