期刊论文

中文

中药新药与临床药理

1003-9783

2015

26

5

613-618

10.3969/j.issn.1003-9783.2015.05.010

新药研发一企业委托课题：中药六类新药“补肾疗更浸膏”临床前新药开发药效学研究.

本校为第一机构

目的研究补肾疗更浸膏延缓老年小鼠卵巢功能衰退作用及其作用机制。方法选取12~13月龄自然衰老小鼠60只,随机分为6组,每组10只,即老年空白组,戊酸雌二醇片组(0.00026 g·kg~(-1)),坤泰胶囊组(1.56g·kg~(-1)),补肾疗更浸膏低、中、高剂量组(6.5,13.0,26.0 g·kg~(-1)),另取3月龄小鼠作为青年对照组。末次给药24 h后,采用酶联免疫吸附法(ELISA)检测小鼠血清雌二醇(E2)、促黄体生成素(LH)、促卵泡激素(FSH)、孕酮(P)含量;取小鼠子宫、卵巢,称质量并计算脏器指数;10%甲醇固定,观察子宫、卵巢病理学改变;采用DNA末端原位标记法(TUNEL)测定小鼠卵巢颗粒细胞凋亡数。结果补肾疗更浸膏可升高老年小鼠血...

Objective To study the effect of Bushen Liaogeng Extract（BLE） on delaying ovarian failure in the aged mice and to explore the mechanism. Methods Sixty mice aged 12-13 months were randomly divided into natural aging control group, model group, estradiol valerate tablets group （0.00026 g·kg-1）, Kuntai capsule group（ 1.56 g·kg-1）, and low-, middle- and high-dose BLE groups（in the dosage of 6.5, 13, 26 g·kg-1 respectively）. Mice aged 3 months served as the youth control group. Twenty-four hours after the last administration, we used enzy...