期刊论文

Effect of Shuwei Decoction on Small Intestinal Propulsive Function and CNP/cGMP Signaling Pathway in Rats with Functional Dyspepsia (FD)

中文

中药新药与临床药理

1003-9783

2019

30

3

276-281

10.19378/j.issn.1003-9783.2019.03.002

国家自然科学基金青年项目(81403384) 国家自然科学基金面上项目(81373577) 湖南省自然科学基金项目(2017JJ3248) 湖南省教育厅优秀青年项目(15B181).

本校为第一机构

目的探讨舒胃汤对功能性消化不良(FD)模型大鼠小肠推进功能及C-钠尿肽(CNP)/环磷酸鸟苷(cGMP)信号通路的影响及机制。方法取80只Wistar大鼠,按体质量随机分为空白组、模型组、舒胃汤组(30.68 g·kg~(-1))、莫沙必利组(1.37 mg·kg~(-1))。采用改良复合病因方法(慢性束缚应激+过度疲劳+饮食失节+夹尾激怒)复制FD模型,造模结束后,连续灌胃给药14 d。采用碳末法检测小肠推进率;多道生理记录系统检测CNP(1×10~(-8) mol·L~(-1))对大鼠离体胃窦肌条的收缩抑制率;ELISA法检测胃窦组织中cGMP含量;Western Blot法检测胃窦组织B型钠尿肽受体(NPR-B)蛋白含量;RT-PCR法检测胃窦平滑肌NPR-B mRNA表达。结果...

Objective To explore the mechanism of Shuwei decoction in the treatment of functional dyspepsia(FD) by studying the effect on Shuwei decoction on intestinal propulsion and CNP/cGMP signaling pathway in rats with FD. Methods Eighty Wistar rats were randomly divided into blank group, model group, Shuwei decoction group (30.68 g·kg~(-1)) and Mosapride group (1.37 mg·kg~(-1)). Except the blank group,the other groups of animals were treated with improved complex pathogeny (chronic restraint stress+excess fatigue+improper diet+tail clamping stimula...