期刊论文

The Regulation of Bushen-Follicle Recuperation Therapy on Expression of AMH Protein and Receptor in PCO Model Rats

中文

中药新药与临床药理

1003-9783

2019

30

11

1314-1321

10.19378/j.issn.1003-9783.2019.11.007

国家自然科学基金资助项目(81503611) 湖南省自然科学基金资助项目(2015JJ6078) 湖南省教育厅优秀青年基金资助项目(15B180)。

本校为第一机构

目的研究补肾调泡周期法对多囊卵巢(PCO)模型大鼠卵巢抗苗勒氏管激素(AMH)及II型受体(AMHR II)蛋白表达的调控,初步探讨补肾调泡周期法治疗多囊卵巢综合征(PCOS)的作用机制。方法110只SD雌性大鼠随机选取10只作为正常组,剩余100只采用来曲唑溶液灌胃法构建PCO大鼠模型成功后,设立模型组、阳性对照组、A-逍遥散(高、低剂量)组、B-三子汤(高、低剂量)组、A+B-逍遥散+三子汤周期用药4组,每组10只。统计分析各组大鼠治疗前后体质量增加量;卵巢脏器系数;HE染色法观察卵巢组织病理形态;免疫组织化学法检测PCO大鼠卵巢AMH、AMHRⅡ蛋白表达。结果体质量增加量:与正常组比较,PCO模型组大鼠体质量增加量明...

Objective To study the regulation of Bushen- follicle recuperation therapy on the expression of AMH Protein and type II receptor(AMHRⅡ)expression in PCO rat models, to investigate the mechanism of Bushenfollicle recuperation therapy for polycystic ovary syndrome(PCOS). Methods Ten of 110 female SD rats were randomly selected as normal group,the remained 100 rats were divided into model group,positive control group,A-Xiaoyao pulvis high-,and low-dose groups,B-Sanzi decoction high-,and low-dose groups,A+B-Xiaoyao pulvis+Sanzi decoction group,10 ...