期刊论文

Qi-Boosting Toxin-Resolving Formula Promotes Apoptosis of Nasopharyngeal Carcinoma Cells by Inducing Autophagy

中文

中药新药与临床药理

1003-9783

2019

30

10

1149-1158

10.19378/j.issn.1003-9783.2019.10.001

国家自然科学基金项目(81573721、81874408) 湖南省自然科学基金项目(2016JJ6117、2017JJ3246) 湖南省教育厅科研基金项目(16C1212) 湖南中医药大学研究生创新课题项目(2017CX33) 中医药防治眼耳鼻咽喉疾病湖南省重点实验室开放基金项目(2018YZD02).

本校为第一机构

目的研究益气解毒方(Qi-boosting toxin-resolving Formula,QBTRF)对人鼻咽癌CNE-2Z细胞自噬和凋亡的影响,探讨CNE-2Z细胞自噬和凋亡之间的关系。方法CCK-8法检测不同浓度益气解毒方干预鼻咽癌CNE-2Z细胞24 h和48 h后的细胞存活率,并计算48 h的IC_(50);采用Annexin V-FITC/PI流式细胞术Western Blot和透射电镜等方法检测QBTRF干预鼻咽癌CNE-2Z细胞后的自噬和凋亡情况。结果CCK-8法结果显示,QBTRF能显著抑制CNE-2Z细胞增殖(P

Objective To study the effect of Qi-boosting Toxin-resolving formula(QBTRF) on autophagy and apoptosis of CNE-2Z cells of human nasopharyngeal carcinoma,and to explore the relationship between autophagy and apoptosis of CNE-2Z cells. Methods CCK-8 assay was used to detect the cell viability of nasopharyngeal carcinoma CNE-2Z cells treated with different concentrations of QBTRF for 24 h and 48 h,and IC5(0 Half Inhibitory Rate Concentration) was calculated at 48 h. Annexin V-FITC/PI, Western Blot and transmission electron microscope were used to ...