期刊论文

Cloning and Bioinformatics Analysis of Squalene Synthase(KcSQS)Gene from Kadsura coccinea (Lem.) A.C.Smith Based on Transcriptome Sequencing

中文

中药新药与临床药理

1003-9783

2019

30

7

870-878

10.19378/j.issn.1003-9783.2019.07.019

广州市科技计划项目(2018-1002-SF-0437) 广州中医药大学2017年度“高水平大学建设”“杏林青年学者”项目(A1-AFD018171Z0311).

本校为第一机构

目的研究黑老虎[Kadsura coccinea(Lem.)A. C. Smith]转录组特征,克隆三萜生物合成上游途径的关键酶基因角鲨烯合酶(KcSQS),为黑老虎资源利用奠定基础。方法对黑老虎不同部位进行转录组测序分析,根据筛选出的KcSQS候选基因序列设计引物,利用PCR技术进行基因克隆,并借助生物学软件进行生物信息学分析。结果克隆得到一个KcSQS基因,包含全长1 227 bp的开放阅读框(ORF),编码408个氨基酸,预测其蛋白分子量为46.65 kD,定位于细胞质膜,含一段跨膜超螺旋结构。该基因在黑老虎叶片中表达量最高,与同科植物厚朴(Magnolia officinalis Rehd. et Wils.)角鲨烯合酶(MoSQS)蛋白序列相似性为76.77%,预测为角鲨烯...

Objective Studying the transcriptome feature and cloning the gene encoding key upstream enzyme of squalene synthase(SQS)of Kadsura coccinea(Lem.)A.C.Smith.This study will contribute the utility of K.coccinea(Lem.)A.C.Smith resource.Methods Transcriptome sequencing was performed on different organs of K.coccinea(Lem.)A.C.Smith.The primers were designed according to the candidate KcSQS unigene sequences,and the gene was cloned by PCR technology.The biological information of KcSQS was analyzed using biological software.Results The KcSQS gene contains a 1227 bp Open Reading Frame(ORF),encodes 408 ...