期刊论文

The Effect of Jingui Shenqi Decoction on Oxidative Stress and Autophagy in Rats with Unilateral Ureteral Obstruction

中文

中药新药与临床药理

1003-9783

2020

31

8

906-911

10.19378/j.issn.1003-9783.2020.08.005

国家自然科学基金青年项目(81603470) 湖南中医药大学一级学科基础医学建设项目(No.06)。

本校为第一机构

目的探讨金匮肾气汤对单侧输尿管梗阻(Unilateral ureteral obstruction,UUO)大鼠肾间质纤维化中氧化应激和自噬的影响。方法将24只雄性SD大鼠随机分为假手术组、模型组、金匮肾气汤组(1.8 g·kg~(-1))、依那普利组(10 mg·kg~(-1));左侧输尿管结扎术后第1天开始灌胃给药(10 mL·kg~(-1)),每日1次,第21天处死大鼠。取梗阻肾组织,采用Masson染色法观察肾组织病理改变;化学荧光法检测梗阻肾组织活性氧(ROS)含量;Western Blot法检测NOX4、p22~(phox)、p47~(phox)、LC3II/I、p62、p-mTOR/mTOR及p-AMPK/AMPK等蛋白在梗阻肾组织中的表达。结果与假手术组比较,模型组大鼠梗阻肾组织间质胶原沉积增多,RO...

Objective To investigate the effect of Jingui Shenqi decoction on oxidative stress and autophagy in unilateral ureteral obstruction(UUO)rats.Methods Twenty-four male SD rats were randomly divided into 4 groups:Sham operation group,model group,Jingui Shenqi decoction treatment group(1.8 g ·kg~(-1)),and the enalapril treatment group(10 mg ·kg~(-1))as positive control.One day after left ureteral ligation,rats were intragastric administration with drugs(10 mL·kg~(-1))for 21 days.On 21th day,the rats were sacrificed.The obstructive kidney tissues...