期刊论文

Effect of Zicuitongmai Yin on Myocardial Fibrosis and TGF-β1/Smads Signaling Pathway in Diabetic Cardiomyopathy Rats

中文

中药新药与临床药理

1003-9783

2021

32

01

29-35

10.19378/j.issn.1003-9783.2021.01.004

湖南省自然科学基金项目（2016JJ6087）；

本校为第一机构

目的探讨滋膵通脉饮对糖尿病心肌病大鼠转化生长因子(TGF)-β1/Smads3信号通路的影响。方法将SD大鼠随机分为空白对照组,模型组,滋膵通脉饮高、低剂量组(17.6 g·kg~(-1),8.8 g·kg~(-1))和依那普利组(0.53 mg·kg~(-1)),空白对照组予以腹腔注射等量柠檬酸-柠檬酸三钠缓冲液,余组大鼠采用高脂高糖饲料喂养+单次腹腔注射链脲佐菌素的方法构建2型糖尿病大鼠模型。造模结束后,模型组和空白对照组灌服同等容积的蒸馏水,余组分别给与药物干预,共干预4周。4周后观察心脏指数和左室指数,PCR技术检测大鼠心肌组织转化生长因子(TGF)-β1 mRNA、Smads3 mRNA和Smads7 mRNA的表达,蛋白印迹法检测心肌组织TGF...

Objective To explore the effect of Zicuitongmai Yin on TGF-β1/Smads signal pathway in diabetic cardiomyopathy rats.Methods SD rats were randomly divided into blank control group,model group,high and low dose group(17.6 g·kg~(-1),8.8 g·kg~(-1))and enalapril group(0.53 mg·kg~(-1)).The blank control group was given the same amount of citric acid-trisodium citrate buffer by intraperitoneal injection,and high fat and high sugar diet plus a single intraperitoneal injection of streptozotocin were used to build the model of type 2 diabetic rats.Aft...