期刊论文

Fangguo Lu

英文

Microchimica Acta

0026-3672

2021

188

8

1-10

10.1007/s00604-021-04933-8

National Natural Science FoundationNational Natural Science Foundation of China (NSFC) [81803964, 82074250, 81774126, 81973670]; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2018 M630906, 2019 T120707]; Natural Science Foundation of Hunan ProvinceNatural Science Foundation of Hunan Province [2020JJ4465, 2020JJ4063]; Key Subjects of Hunan University of Chinese Medicine [1]; Basic Medicine Construction Project of Hunan University of Traditional Chinese Medicine [6]; Excellent Teaching Team of Postgraduate in Hunan Province [[2019] 370-118]; Hunan Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine [2019TP1044]

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A graphene-based bioassay is describedfor the fluorometric determination of agrD gene transcription (mRNA) in methicillin-resistant Staphylococcus aureus (MRSA). This method includes exonuclease III (Exo III)-assisted target recycling and DNA walker cascade amplification. Hairpin1 (HP1) consists of a capture probe (CP) and DNA walker sequence. In the absence of the target, 5′-amino modified hairpin2 (HP2) labeled with carboxyfluorescein (FAM) at its 3′ terminus is covalently linked to graphene via 1-ethyl-3-(3-dimethylaminopropyl) carbodiimid...