期刊论文

中文

医学研究杂志

1673-548X

2019

48

1

103-107

10.11969/j.issn.1673-548X.2019.01.024

湖南省研究生创新基金资助项目(CX2016B381).

本校为第一机构

目的探讨精子相关抗原9(SPAG 9)在肝癌细胞HepG 2中表达对HepG 2增殖分化及凋亡的影响,并初步揭示SPAG 9与JNK信号通路的关系。方法采用细胞免疫荧光检测SPAG 9在HepG 2中的表达及定位,siRNA技术敲低SPAG 9的表达,Western blot法检测转染后SPAG 9及JNK蛋白的表达,CCK8(cell counting kit-8)实验检测肿瘤细胞的增殖能力,流式细胞术检测肿瘤细胞的周期及凋亡。结果SPAG 9在HepG 2中表达,定位于细胞质和细胞核。与对照组细胞比较,转染SPAG 9 siRNA使JNK蛋白的表达下调,差异有统计学意义(P

Objective To explore the expression of SPAG 9 in hepatoma HepG 2 cell line and its effects on cell proliferation,differentiation and apoptosis,and reveal the relationship between SPAG 9 and JNK signal pathway.Methods The expression and location of SPAG 9 in HepG 2 was detected by cell immunofluorescence.SiRNA transfection was used to knockdown the expression of SPAG 9.Western blot was used to detect the expression of SPAG 9 and JNK protein after siRNA transfection.Cell Counting Kit-8 assay was used to detect the effect of SPAG 9 on the proliferation of HepG 2 after siRNA transfection.Flow cyto...