期刊论文

中文

中国中医药信息杂志

1005-5304

2015

22

2

55-59

10.3969/j.issn.1005-5304.2015.02.016

国家自然科学基金（81001522） 湖南省自然科学基金（13JJ4138） 首届中国中西医结合学会-金港榄香烯中医药肿瘤研究基金（2010-F10）

本校为第一机构

目的:探讨益气化瘀解毒方及其单味药对人肝癌移植瘤血管拟态相关因子表达的影响。方法瘤细胞接种建立人肝癌移植瘤模型,随机分为益气化瘀解毒方组(全方组)、黄芪组、莪术组、重楼组、壁虎组、顺铂组及模型组,除模型组外,各给药组予相应药物干预。21 d后,采用免疫组化法检测瘤组织低氧诱导因子-1α(HIF-1α)、基质金属蛋白酶(MMP)-9、MMP-2、E-钙黏附蛋白(E-cad)表达,免疫荧光法观察血管生成拟态结构,实时荧光定量 PCR 检测瘤组织 Twist1、Bcl-2表达。结果与模型组比较,给药后各给药组瘤体积减小(P

Objective To observe the influencing of Yiqi Huayu Jiedu Prescription on the growth of HepG2 nude mice transplantation tumor and the expression of related factors of vascular mimicry. Methods Models of transplanted tumors, which were made by HepG2 cells in nude mice, were randomly divided into 7 groups, Yiqi Huayu Jiedu Prescription group, Astragali Radix group, Curcumae Rhizoma group, Paridis Rhizoma group, Gecko group, cis-platinum group, and model group. Except for the model group, the rest groups were given relevant medicine for interventio...