期刊论文

中文

临床肿瘤学杂志

1009-0460

2010

15

9

774-778

10.3969/j.issn.1009-0460.2010.09.002

湖南省科技厅资助项目（2007TP4021） 湖南省教育厅资助项目（08C637、09C724）

本校为第一机构

目的观察壮骨镇痛胶囊对高转移人乳腺癌MDA-MB-231细胞抑癌基因nm23-H1和原癌基因c-myc表达的影响,探讨壮骨镇痛胶囊抑制MDA-MB-231细胞增殖和迁移的可能作用机制。方法采用RT-PCR法和免疫细胞化学法分别检测不同浓度壮骨镇痛胶囊含药血浆对MDA-MB-231细胞nm23-H1和c-myc基因mRNA及其蛋白表达的影响,并分析其对nm23-H1/c-myc蛋白比值的影响。结果 5%壮骨镇痛胶囊含药血浆显著抑制了MDA-MB-231细胞nm23-H1和c-myc基因的转录和翻译;1.25%壮骨镇痛胶囊含药血浆抑制了nm23-H1的转录和翻译以及c-myc基因的翻译,对c-myc基因的转录无显著影响;0.63%壮骨镇痛胶囊含药血浆对nm23-H1和c-myc基因的转录和翻...

Objective In order to provide evidence of mechanisms of Zhuanggu Zhentong capsules,we investigated the impacts of Zhuanggu Zhentong capsules on the expressions of nm23-H1 gene and c-myc gene of breast cancer cell lines MDA-MB-231 in vitro.Methods The mRNA levels of nm23-H1 gene and c-myc gene were examined by reverse transcriptase PCR（RT-PCR）.The nm23-H1 and c-myc proteins were assayed by immunocytochemistry.The ratio of nm23-H1/c-myc proteins was also analyzed.Results The transcriptions and translations of nm23-H1 and c-myc genes of MDA-MB-2...