期刊论文

Comparative Study between Medicated Plasma and Medicated Serum of Yang-Tonifying Five-Returning Decoction in Expression of BMECs NO,v WF,6-keto-PGF1a of Hypoxia Injured Rats and Vivo Experiments

中文

世界中医药

1673-7202

2016

11

4

604-608

10.3969/j.issn.1673-7202.2016.04.009

国家自然科学基金项目（编号:81274008）； 湖南省科研条件专项课题（编号:2011TT2050）； 湖南省科技厅一般项目（编号:2014SK3052）；

本校为第一机构

目的:比较补阳还五汤含药血清和含药血浆对缺氧培养的大鼠脑微血管内皮细胞(BMECs)分泌NO、vWF、6-keto-PGF1α的水平与体内实验真实值的接近程度,为半体内实验选择含药血浆还是含药血清提供参考依据.方法:以氧剥夺BMECs模型为研究对象,观察补阳还五汤含药血浆和含药血清对BMECs条件培养液中一氧化氮(NO)、血管性假血友病因子(vWF)、6-酮-前列腺素1 α (6-keto-PGF1 α)含量的影响,并与补阳还五汤干预的中动脉阻塞模型大鼠血浆上述指标水平进行比较.结果:结果发现:与空白血浆组合空白血清组比较,补阳还五汤含药血浆和含药血清均能明显降低氧剥夺BMECs条件培养液中vWF的含量,升高NO、6-keto-PGF1...

Objective： To compare the approaching degree of the expression of NO,v WF and 6-keto-PGF1 a between Yang-Tonifying Five-Returning Decoction（ YFD） contained plasma and serum on brain microvascular endothelial cells（ BMECs） injured by hypoxia rats with true value of animals in vivo experiments,and to provide references for selecting plasma or serum in the study of ex vivo experiments. Methods： The model of MCAO was made by Suture Embolization Method in the internal carotid artery of rats.BMECs were made in hypoxia incubator. The method of E...