期刊论文

Research on the expression of peripheral blood mononuclear cell HLA-G in patients with hepatocellular carcinoma

中文

检验医学

1673-8640

2013

12

1088-1091

10.3969/j.issn.1673-8640.2013.12.006

本校为第一机构

目的：了解肝细胞癌（HCC）患者外周血单个核细胞（PBMC）人类白细胞抗原G（HLA-G）的表达水平。方法收集38例HCC患者、25例肝硬化患者和40名健康查体者的PBMC，分别用逆转录-聚合酶链反应（PCR）和流式细胞术（FCM）检测其HLA-G mRNA和HLA-G蛋白表达水平。结果 HCC组、肝硬化组和健康对照组PBMC HLA-G mRNA表达率分别为94．7％、84．0％和87．5％，差异无统计学意义（P＝0．359）；HCC组HLA-G蛋白表达率高于肝硬化组和健康对照组，差异有统计学意义（P＝0．002）。HCC组PBMC HLA-G蛋白主要表达于CD4＋细胞上。结论 HCC患者PBMC HLA-G蛋白表达率升高，其机制与临床意义尚待进一步研究。

Objective To investigate the expression level of peripheral blood mononuclear cell （PBMC） human leucocyte antigen G （HLA-G） in patients with hepatocellular carcinoma （HCC）. Methods The HLA-G mRNA and HLA-G protein expression levels on PBMC from 38 patients with HCC, 25 patients with fiver cirrhosis and 40 healthy subjects were determined by reverse transeription-polymerase chain reaction （PCR） and flow cytometry （FCM）. Results The HLA-G mRNA expression rates in HCC group, cirrhosis group and healthy control group were 94.7% ,84.0% and...