期刊论文

中文

四川中医

1000-3649

2017

35

12

38-41

湖南省教育厅高校科研项目（编号:15C1034）；

本校为第一机构

目的:通过补阳还五汤水提醇沉液的指纹图谱,分析其对大鼠急性脑缺血神经细胞损伤及凋亡的谱效学关系。方法:选择参照改良栓线制备脑缺血且评定得分>7的大鼠共90只,随机分为8个实验组和1个对照组,按要求灌胃。收集灌胃后各组大鼠含药血浆、脑匀浆液,比较灌胃后各组大鼠脑组织TTC染色情况,分别采用柱前衍生HPLC法和ELISA试剂盒法测定谷氨酸和生理指标水平并记录分析。分析各组大鼠血浆指纹图谱主要成份与生理指标的逻辑关系及体内外指纹图谱最大效应成分群。结果:对各组大鼠脑组织TTC染色情况进行比较,发现灌胃补阳还五汤的大鼠相对于对照组差异明显,差异有统计学意义（P0.05）;认定成分12、19、7...

Objective： To analyze the spectral relationship between the injury and apoptosis of cerebral ischemic neurons by the fingerprints of alcohol extract of Buyang Huanwu Decoction. Methods： A total of 90rats were randomly divided into 8experimental groups and one controlled group. The TTC staining of the brain tissue of the rats after intragastric administration was collected and the levels of glutamate and physiological indexes were determined by HPLC method and ELISA method. And record the analysis. The logarithmic relationship between the main...