期刊论文

中文

吉林中医药

1003-5699

2015

35

1

53-56

10.13463/j.cnki.jlzyy.2015.01.016

湖南省研究生创新基金项目（CX2011B342）；

本校为第一机构

目的探讨六神丸对食管癌移植瘤组织细胞凋亡与B淋巴细胞瘤-2基因（Bcl-2）表达的影响及相关机制。方法通过皮下注射细胞悬液建立食管癌移植瘤模型,将48只裸鼠随机分为空白组、模型组、顺铂组（DDP）、六神丸高剂量组、六神丸中剂量组、六神丸低剂量组,按照给药方案用药,用药20 d后处死动物,剥取瘤组织,TUNEL（原位末端标记法）检测瘤体组织细胞凋亡指数,免疫组化法检测瘤体组织Bcl-2的表达。结果六神丸高、中、低剂量各组细胞凋亡指数均高于模型组,给药各组瘤体组织内Bcl-2的表达活性均明显下降。结论六神丸通过下调肿瘤组织Bcl-2表达活性、促进细胞凋亡是六神丸抑瘤作用机制之一。

Objective To investigate the effect of Liushen Pill on the apoptosis index and the expression of bcl-2 in esophagus carcinoma xenografts. Methods The model of esophagus carcinoma xenografts in nude mice was established by hypodermic injection with cell suspension. These mice were randomly divided into 6 groups as follows： blank group,model group,DDP group,high dosage of Liushen Pill group,middle dosage group,low dosage group. The models were treated by dosage regimen. These mice were sacrificed after 20 days treatment and the tumor tissue was ...