期刊论文

Ning, Yi（宁毅）

英文

Microchimica Acta

0026-3672

2017

184

10

3875-3882

10.1007/s00604-017-2363-4

National Natural Science Foundation of Hunan provinceNatural Science Foundation of Hunan Province [2016JJ3098]; Outstanding Youth Scientific Research Project - Education department of Hunan Province [15B169]; National Natural Science FoundationNational Natural Science Foundation of China (NSFC) [81202451]; Science and Technology Innovation Team in Colleges and Universities in Hunan Province "Chinese traditional medicine for treatment of infectious diseases" [15, Grxjb-7]; Doctor start-up Foundation of Hunan University of Chinese Medicine [9982-1001019]; Key Subjects of Hunan University of Chinese Medicine "pathogenic biology" [1]; Hunan Provincial Level Course "Immunology and pathogenic biology" [48]

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The authors describe a low-cost and sensitive method for the fluorometric determination of Salmonella enteritidis (S. enteritidis). It is based on the use of nanographite (NG) as a quencher of fluorescence, and on cyclic signal amplification by using deoxyribonuclease I (DNase I). The probe containing a capture probe and two short sequences (probe 1 and probe 2) labeled with carboxyfluorescein (FAM) are used as the signal probe that is adsorbed on the surface of NG via p-stacking interactions. Adsorption results in quenching of the fluorescence of FAM. If S. enteridis is introduced, fluorescen...