期刊论文

Ning, Y

英文

Microchimica Acta

0026-3672

2017

184

10

3875-3882

10.1007/s00604-017-2363-4

We would like to thank National Natural Science Foundation of Hunan province(2016JJ3098), Outstanding Youth Scientific Research Project Funded by Education department of Hunan Province (15B169), National Natural Science Foundation (81202451), Science and Technology Innovation Team in Colleges and Universities in Hunan Province《Chinese traditional medicine for treatment of infectious diseases》(No: 15, Grxjb-7), Doctor start-up Foundation of Hunan University of Chinese Medicine (9982-1001019), Key Subjects of Hunan University of Chinese Medicine《pathogenic biology》(NO.1) and Project Funded by Hunan Provincial Level Course《Immunology and pathogenic biology》(No. 48) for the financial support.

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The authors describe a low-cost and sensitive method for the fluorometric determination of Salmonella enteritidis (S. enteritidis). It is based on the use of nanographite (NG) as a quencher of fluorescence, and on cyclic signal amplification by using deoxyribonuclease I (DNase I). The probe containing a capture probe and two short sequences (probe 1 and probe 2) labeled with carboxyfluorescein (FAM) are used as the signal probe that is adsorbed on the surface of NG via p-stacking interactions. Adsorption results in quenching of the fluorescence...