期刊论文

Effects of Panax notoginseng saponins pretreatment on expressions of NF-kappa Bp65 and IL-1β in rats with renal ischemia reperfusion injury

中文

中药药理与临床

1001-859X

2017

33

5

58-62

10.13412/j.cnki.zyyl.2017.05.016

中西医结合基础湖南省＂十二五＂重点学科开放基金资助项目（项目编号ZXJCXK201503） 湖南省大学生研究性学习和创新性实验计划项目（项目编号2015-216） 国家自然科学基金青年项目（项目编号81503442 81603470）

本校为第一机构

目的:探讨不同剂量三七总皂苷预处理后对大鼠肾缺血再灌注损伤NF-κB信号通路的影响。方法: SD大鼠40只,随机分为空白对照组,肾缺血再灌注损伤组,三七总皂苷40、80、160 mg /kg组,前两组给予生理盐水,后3组分别给予三七总皂苷40、80、160 mg /kg连续给药5天。后4组在最后1次给药1h后通过切除右肾、夹闭左肾动脉45min再灌注3h的方法制备肾缺血再灌注损伤模型。术后处死大鼠,检测血肌酐、尿素氮,HE染色观察肾组织病理学改变,免疫组化法和PT-PCR法分别检测核因子- κBp65、白细胞介素-1β蛋白水平和mRNA表达。结果:肾缺血再灌注损伤组BUN、SCr水平明显增高,光镜下可见肾小管损伤明显,肾组织NF-κBp...

Objective: To investigate the protective effects of Panax Notoginseng Saponins pretreatment on nuclear factor-kappa B signaling pathway in rats with renal ischemia-reperfusion injury. Methods: 40 SD rats were randomly divided into 5 groups as follow: the control group,the renal ischemia reperfusion injury group,40,80,160 mg /kg of Panax Notoginseng Saponins groups. Mice in the former two groups were given saline,and mice in the latter three groups were given 40,80,160 mg /kg Panax Notoginseng Saponins for 5 day. In the latter four groups, the m...