期刊论文

中文

中医正骨

1001-6015

2024

36

02

17-22+31

10.3969/j.issn.1001-6015.2024.02.004

B2023153:湖南省中医药科研课题 QZYY-2019-031:贵州省中医药管理局中医药、民族医药科学技术研究课题

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目的:观察共培养条件下肌卫星细胞C2C12对软骨细胞ATDC5活性的影响。方法:（1）将肌卫星细胞C2C12分为对照组和地塞米松组，对照组常规培养，地塞米松组采用地塞米松干预，通过CCK8法和BCA法测定地塞米松对C2C12细胞增殖和蛋白合成的影响，以划痕实验和Transwell小室观察地塞米松对C2C12细胞修复和迁移能力的影响。（2）采用Transwell小室将正常肌卫星细胞C2C12（共培养组）和经地塞米松预处理的肌卫星细胞C2C12（预处理组）分别与软骨细胞ATDC5共培养，对照组Transwell下层小室内接种ATDC5细胞、上层小室内为无细胞的常规培养基，采用CCK8法和二氯二氢荧光素-乙酰乙酸酯荧光探针检测ATDC5细胞增...

Objective:To observe the effect of skeletal muscle satellite cells(MuSCs)C2C12 on the activity of chondrocytes ATDC5 in the case of co-cultured condition.Methods:①The C2C12 cells were cultured,and the third-generation cells were collected and divided into control group and dexamethasone(DEX)group.The C2C12 cells in the control group were cultured in the conventional Dulbecco's Modified Eagle's Medium(DMEM),while the ones in DEX group were intervened with DEX.After that,the effects of DEX on proliferation and pro-tein synthesis in C2C12 cells w...