期刊论文

中文

药物评价研究

1674-6376

2018

41

12

2214-2220

10.7501/j.issn.1674-6376.2018.12.015

GTZK201607:天然药物活性物质与功能国家重点实验室开放基金 :一流学科建设项目 2015B172:湖南省教育厅优秀青年项目 201494:湖南省中医药科研项目 2015C1039:湖南省教育厅一般项目 1007:湖南省十二五省级药学重点学科开放基金项目

本校为第一机构

目的 进行鱼腥草注射液与新鱼腥草素钠注射液体外人肝微粒体代谢研究,为进一步阐明中药多成分体系体内代谢规律奠定基础.方法 制备人肝微粒体,用Brad ford法测定酶活性,配制肝微粒体体外温孵体系,加入鱼腥草注射液与新鱼腥草素钠注射液温孵,用气相色谱-质谱联用(GC/MS)法测定并比较鱼腥草注射液及新鱼腥草素钠注射液代谢样品图谱.结果 检测到鱼腥草注射液的人肝微粒代谢产物20个,新鱼腥草素钠注射液肝微粒代谢产物24个,鱼腥草注射液多成分体系与新鱼腥草素钠注射液单成分经肝微粒体代谢其产物的数目不完全相同,但产物结构类型一致,均为脂肪族、芳香族、萜类挥发性成分,其中两者的代谢产物中均有...

Objective Studying the metabolism of Houttuyniae and sodium new Houttuyfonate injection in liver microsomes to lay a foundation for clarifying the metabolism rule of Chinese herbal multi components system in vivo and Determining the Q-Marker of houttuynia.Methods The liver microsome was prepared and the enzymatic activity was determined by Brad ford method.The in vivo iver microsomes incubating system was prepared,and the Houttuyniae injection and sodium new Houttuyfonate injection were incubated in the liver microsomes;the metabolic product were determined by GC-MS.Results Houttuynia injectio...