期刊论文

Analysis of the Structure and Function of S Protein of 2019 Novel Corona Virus

中文

微生物学杂志

1005-7021

2020

40

03

41-50

10.3969/j.issn.1005-7021.2020.03.007

2019年湖南省教育厅科学研究项目优秀青年项目（19B436）； 湖南中医药大学“十三五”冶一级学科基础医学建设项目（06）；

本校为第一机构

运用生物信息学,预测急性呼吸系统综合症冠状病毒2(SARS-CoV-2/2019-nCoV)的基本理化性质、结构、功能和抗原表位等,为新型冠状病毒肺炎(COVID-19)的防治提供思路。应用ExPASy分析S蛋白的消光系数、不稳定系数和半衰期等理化性质;利用SignaIP v5.0分析S蛋白的信号肽;应用TMHMM分析S蛋白的跨膜区;利用NetPhos3.1在线工具预测S蛋白的磷酸化位点;应用Pfam预测S蛋白的结构域;应用PSIPRED分析S蛋白的二级结构特征;利用SWISS-MODEL构建S蛋白的三级结构;利用BLAST分析SARSCoV-2的S蛋白与其他物种的相似性;利用MEGA软件分析2019-nCoV的S蛋白与其他物种的进化关系。S蛋白由1 273个氨基酸组成,其相对分子质...

Basic physicochemical properties,structure,function and antigen epitope of severe acute respiratory syndrome corona virus 2(SARS-Cov-2/2019-nCov)were predicted using bioinformatics,to provide train of thought to contain COVID-19.The extinction coefficient,instability coefficient,half-jlife,and other physicochemical properties of S protein were analyzed applying ExPASy;The signal peptide were analyzed using SignaIP v5.0;and applying TMHMM to analyze transmembrane region of S protein;The phosphorylation sites were predicted adopting on-line tool NetPhos3.1;The structural domain of S protein was ...