期刊论文

Structural and Functional Characteristics Analysis of SARS-CoV-2 3CL~(pro) via Bioinformatics

中文

微生物学杂志

1005-7021

2020

40

6

44-52

10.3969/j.issn.1005-7021.2020.06.007

2019年湖南省教育厅科学研究项目优秀青年项目（19B436）； 湖南中医药大学“十三五”一级学科基础医学建设项目（No.06）；

本校为第一机构

采用生物信息学方法分析新型冠状病毒(Severe acute respiratory syndrome coronavirus 2,SARS-CoV- 2)3C样蛋白酶(3-chymotrypsin-like protease,3CL~(pro) )的理化性质、结构与功能,为抗SARS-CoV-2药物研发提供参考。通过ProtParam、ProtScale、Bioedit服务器对3CL~(pro)进行一级结构如氨基酸理化性质、疏水性的预测分析;COILS Server、SignalP、TMPred、TargetP Server、NetPhos Server、NetNGlyc Server服务器对3CL~(pro)结构进行如卷曲螺旋区、信号肽、跨膜结构域、亚细胞定位、磷酸化位点、糖基化位点的预测分析;SOPMA、SWISS-MODEL服务器对3CL~(pro)进行二级结构、三级结构的预测分析;IE...

In this study,physicochemical properties,structure and function of 3-chymotrypsin-like protease(3CL~(pro) )were predicted via bioinformatics,to provide references for R &D of anti-SARS-CoV-2 medicines.By predicting and analyzing the primary structure of 3CL~(pro) such as physicochemical properties of amino acids,hydrophobic characteristics prediction analysis in terms of theoretical isoelectric point,half-life period,fat coefficient and hydrophobic characteristics of 3CL~(pro) on ProtParam,ProtScale and Bioedit;the structure such as curling spiral region on the COILS Server, signal peptides on...