期刊论文

Delayed Protection of Ischemic Preconditioning against Myocardial Ischemia-reperfusion Injury in Rabbits

中文

中国医师杂志

1008-1372

2004

6

2

200-203

10.3760/cma.j.issn.1008-1372.2004.02.023

湖南省自然科学基金

本校为第一机构

目的探讨缺血预处理对家兔缺血再灌注损伤心肌的延迟保护作用.方法 24只健康家兔,随机分为假手术组(Sham)、缺血再灌注组(I/R)和缺血预处理组(IP),每组8只,分别预处理24h后行心肌缺血60min,再灌注120min,观察缺血心肌形态结构变化、缺血区面积和危险区面积、血清腺苷(Adenosine)、降钙素基因相关肽(CGRP)含量.结果 I/R家兔部分心肌纤维断裂,有灶性出血,心肌间质大量炎性细胞浸润,血清腺苷和CGRP含量显著下降(P＜0.01);缺血预处理组部分心肌纤维呈波浪状,肌浆分布不匀,心肌间质无出血,有数量不等炎性细胞浸润,但心肌结构基本正常,仅少数线粒体有轻度水肿;并能显著缩小缺血再灌注损伤家兔心肌梗死...

Objective To explore the delayed protection of ischemic preconditioning against myocardial ischemia-reperfusion injury in rabbits. Methods Twenty four healthy rabbits were randomly into sham, ischemia-reperfusion(I/R) and ischemic preconditioning(IP) groups, each group containing 8 rabbits. After 24h IP, rabbits in IP group received 60 min coronary artery occlusion followed by 120 min reperfusion. Myocardial morphological changes were observed by optics microscope and electron microscope, infarction size and the area at risk were determined by triphenyltetrazolium chloride(TTC) and Evan's blue...