期刊论文

Protective effect of dexmedetomidine pretreatment on the cardiac muscle cell in myocardial ischemia reperfusion of rat

中文

中国临床药理学杂志

1001-6821

2017

33

20

2032-2034

10.13699/j.cnki.1001-6821.2017.20.011

本校为其他机构

目的研究右美托咪定(Dex)预处理对缺血再灌注大鼠心肌细胞凋亡及凋亡蛋白表达的影响。方法按照体重将雄性SD大鼠随机分为4组,每组7只:低高2个剂量实验组(结扎前0.5 h分别腹腔注射Dex 10, 30 μg·kg~(-1)),模型组及假手术组(结扎前0.5 h均腹腔注射0.9%NaCl 5 mL·kg~(-1))。用结扎左冠状动脉前支构建心肌缺血-再灌注损伤大鼠模型。以缺口末端标记(TUNEL)法检测各组大鼠心肌细胞凋亡率;以免疫组化法检测各组大鼠心肌组织中Bcl-2及Bax蛋白表达情况。结果假手术组、模型组与低高2个剂量实验组的心肌细胞凋亡率(AR,%)分别为0.42 ± 0.06,19.73 ± 4.12,13.31 ± 3.65, 10.04 ± 2.71;这4组的心肌组织...

Objective To explore the protective effect of dexmedetomi- dine （Dex） pretreatment on the cardiac muscle cell in myocardial ische- mia reperfusion of rat. Methods Male rats were divided into 4 groups, 7 cases in each group：experimental -L group, experimental -H group （intraperitoneal injection 10, 30 μg·kg（-1） Dex at the time of 0. 5 h be- fore ligatured） , model group and sham -operation group （intraperitoneal injection 5 μg·kg（-1） 0. 9% NaCI at the time of 0. 5 h before ligatured）. The myocardial ischemia- reperfusion injury ra...