期刊论文

中文

中国临床药理学杂志

1001-6821

2017

33

15

1494-1497

10.13699/j.cnki.1001-6821.2017.15.035

湖南省教育厅重点基金资助项目

本校为第一机构

目的研究分析六味地黄汤水提醇溶部位的主要成分及其补肾作用机制。方法用 HypersileC_(18)(4.6mm×250 mm,5 μm)色谱柱,以甲醇-水(27:73)为流动相,检测波长为236 nm;流速为1.0 mL · min-1;柱温为25℃, 用高效液相色谱法测定六味地黄汤水提醇溶部位的主要成分及含量。按照性别和体重将30只小鼠随机分为3组(n = 10):正常组、模型组、实验组。实验组按9.75 g · kg~(-1)体重灌胃六味地黄汤水提醇溶部位,连续给药9 d;在给药第6天,除正常组外,其他各组按50 mg · kg~(-1)体重灌胃氢化可的松,连续4 d,建立肾阴虚小鼠模型。第10天,用ELISA法测定小鼠血浆环磷酸腺苷(cAMP)、环磷酸鸟苷(cGMP)含量,用放...

Objective To analyze the chemical components of the ethanol -soluble portion of Liuwei Dihuang Decoction, and to explore its mechanism in kidney - Yin deficiency of mice. Methods Bioactive components were analyzed by HPLC, the method was used at Hypersile C_(18) column(4. 6 mm x 250 mm,5 μm),the mobile phase consisted of methanol - water ( 27: 73 ),the detective wavelength was 236 nm, the flow rate was 1. 0 mL · min-1,the column temperature was 25 ℃. The mice were divided randomly into three groups( n = 10) : blank group, model group, experi...