期刊论文

Mechanism of Xuefuzhuyu decoction on the delaying senility of bone marrow derived endothelial progenitor cells in rats

中文

中国临床药理学杂志

1001-6821

2018

34

23

2723-2726

10.13699/j.cnki.1001-6821.2018.23.012

国家自然科学基金面上基金资助项目(81574039).

本校为第一机构

目的 观察血府逐瘀汤对血管紧张素Ⅱ( AngⅡ)诱导大鼠骨髓源内皮祖细胞(EPCs)衰老及肿瘤抑制基因(p53)、沉默信息调节因子1(SIRT1)表达的影响.方法 体外培养EPCs,加入1×10 -7mol· L-1AngⅡ诱导细胞衰老,建立内皮祖细胞衰老模型.将EPCs随机分为6组:正常组(不做处理)、模型组(空白血清),对照组(miR-34a抑制剂)和低、中、高3个剂量实验组(5%,10%,15%含药血清).分别处理24,48,72h后,收集细胞.以β-半乳糖苷酶染色法检测内皮祖细胞衰老状态;以实时荧光定量法检测p53 mRNA、SIRT1 mRNA的表达.结果 正常组、模型组、对照组和低、中、高3个剂量实验组的衰老细胞百分数分别为( 10.67 ± 1.52 )%, ( 66....

Objective To observe the effect of Xuefuzhuyu decoction on the senescence of bone marrow-derived endothelial progenitor cells(EPCs) induced by angiotensin Ⅱ (AngⅡ) and the expression of tumor-suppressing gene (p53) mRNA and silent information regulator 1(SIR1) mRNA in cell senescence,in order to explore its anti-aging effect and mechanism.Methods EPCs were cultured in vitro,and 1×10~(-7) mol·L~(-1)AngⅡwas used to induce cell senescence.The model of EPCs senescence was established.The EPCs were divided into 6 groups:normal group(no treatmen...